CGmCGCG is a versatile substrate with which to evaluate Tet protein activity.

نویسندگان

  • Seiichiro Kizaki
  • Hiroshi Sugiyama
چکیده

Tet family proteins have the ability to convert 5-methylcytosine (mC) to 5-hydroxymethylcytosine, and further to 5-formylcytosine and 5-carboxycytosine. We found that CGmCGCG can be the substrate of Tet protein, and observed iterative oxidation of mC by HPLC analysis. We also demonstrated that Tet protein favours single-stranded DNA over double-stranded DNA.

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عنوان ژورنال:
  • Organic & biomolecular chemistry

دوره 12 1  شماره 

صفحات  -

تاریخ انتشار 2014